Protein aggregates in cytoplasm interfere with important transport routes.
Proteins consist of long chains of amino acids and function in cells like small machines. To be able to fulfill their function proteins have to assume a predetermined three-dimensional structure. In healthy cells there is a large variety of folding helpers and extensive quality control machinery. Misfolded proteins are either repaired or rapidly degraded. If this occurs inadequately, or not at all, proteins will clump together, form aggregates and harm the cell.
Protein aggregates are associated with many neurodegenerative diseases including ALS, Alzheimer’s, Parkinson’s and Huntington’s Disease. How exactly aggregates harm cells is however still unknown. In 2013 several groups in Martinsried formed the ToPAG consortium to address this question, and can now report their first success. Scientists in the lab of Prof. Hartl, a world-renowned expert on protein folding, have demonstrated that the location of the aggregates determines the fate of the nerve cells.
Together with Konstanze Winklhofer and Jörg Tatzelt from the Ruhr-University Bochum, the researchers have expressed artificial aggregation prone proteins as well as Huntington’s disease-causing mutants of the protein huntingtin in cultured cells. Both types of protein accumulate in large protein deposits. “It came as a big surprise to us that the direction of the proteins to the cytoplasm instead of the nucleus resulted in more soluble, but also more toxic aggregates”, says Mark Hipp, a group leader in the department of Ulrich Hartl and leader of the study.
The protein deposits in the cytoplasm blocked the transport of RNA and correctly folded proteins between the nucleus and the cytoplasm. It seems that the sticky surfaces of the aggregates can sequester important proteins and thereby inactivate them. “We have detected multiple components of the cellular transport machinery inside the aggregates. This results in the depletion of these factors from the cell, and, like a machine with missing parts, the cell is then unable to function properly”, explains Andreas Woerner the first author of the study.
Once the blueprint for all proteins, the RNA, is trapped within the nucleus, protein synthesis cannot progress, and the cells deteriorate. It is not completely clear why the nuclear aggregates are less harmful, but the researchers have evidence that the nuclear protein NPM1 plays a central role in shielding these aggregates.
“The results of this study bring us researchers and physicians one big step further”, summarizes Mark Hipp. “Only if we understand how aggregates damage cells is it possible to develop appropriate countermeasures in the future.”
Source: Dr. Christiane Menzfeld – Max Planck Institute
Image Source: The image is credited to MPI of Biochemistry
Original Research: Abstract for “Cytoplasmic protein aggregates interfere with nucleo-cytoplasmic transport of protein and RNA” by A. C. Woerner, F. Frottin, D. Hornburg, L. R. Feng, F. Meissner, M. Patra, J. Tatzelt, M. Mann, K. F. Winklhofer, U. Hartl, and M. S. Hipp in Science. Published online January 9 2016 doi:10.1126/science.aad2033
Cytoplasmic protein aggregates interfere with nucleo-cytoplasmic transport of protein and RNA
Amyloid-like protein aggregation is associated with neurodegeneration and other pathologies. The nature of the toxic aggregate species and their mechanism of action remain elusive. Here, we analyzed the compartment specificity of aggregate toxicity using artificial β-sheet proteins, as well as fragments of mutant huntingtin and TAR DNA binding protein–43 (TDP-43).
Aggregation in the cytoplasm interfered with nucleocytoplasmic protein and RNA transport.
In contrast, the same proteins did not inhibit transport when forming inclusions in the nucleus at or around the nucleolus.
Protein aggregation in the cytoplasm, but not the nucleus, caused the sequestration and mislocalization of proteins containing disordered and low-complexity sequences, including multiple factors of the nuclear import and export machinery.
Thus, impairment of nucleocytoplasmic transport may contribute to the cellular pathology of various aggregate deposition diseases.
“Cytoplasmic protein aggregates interfere with nucleo-cytoplasmic transport of protein and RNA” by A. C. Woerner, F. Frottin, D. Hornburg, L. R. Feng, F. Meissner, M. Patra, J. Tatzelt, M. Mann, K. F. Winklhofer, U. Hartl, and M. S. Hipp in Science. Published online January 9 2016 doi:10.1126/science.aad2033