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Effect of egg white fermentation with lactobacilli on IgE binding ability of egg white proteins; reducing egg allergy by fermentation

The study demonstrated the potential of reducing egg allergy by fermentation of egg white with L. delbrueckii subsp. delbrueckii.
Egg allergy, afflicting around 1.6% to 3.2% of the total children population, is the second most common food allergy among infants and young children. The objective of the study was to determine if lactobacilli fermentation could reduce the IgE binding ability of egg white.

Acidification of egg white to pH6.0 and supplementation of tryptone are necessary to grow lactobacilli in egg white. Cell counts of Lactobacillus sanfranciscensis and Lactobacillus sakei were not affected up to 96h of incubation, while that of Lactobacillus delbrueckii subsp. delbrueckii decreased rapidly at the first 24h of incubation, increased to its inoculated level at 48h, and then leveled off afterwards.

The pH of fermented egg white was reduced to 5 after 48h of incubation with L. sanfranciscensis and/or L. sakei, and after 72h incubation with L. delbrueckii subsp. delbrueckii.
Among three strains studied, only L. delbrueckii subsp. delbrueckii fermented egg white showed 50% reduction in IgE binding ability. No obvious protein degradation in fermented egg white proteins was detected by SDS-PAGE.

The reduction of egg white IgE binding ability was attributed to ovomucoid, the dominant egg allergen, as shown the change of molecular weight analyzed by MALDI-TOF-MS, reduction of intensity of FITC labeled ovomucoid after fermentation, and the change of intensity of glycopeptides containing core+4HexNAc and core+3HexNAc.

Sen Li , Marina Offengenden , Messele Fentabil , Michael G. Gänzle , Jianping Wu. JOURNAL OF MIDWIFERY & WOMEN’S HEALTH, 20131526-9523. June 2013. Food Research International, Volume 52, Issue 1

Published by connie dello buono

Connie Dello Buono is based in Sunnyvale California. Her first ebook is about women's health, Birthing Ways Healing Ways and her recent one is about cancer prevention, Curated Healing Ways. She had helped women have holistic childbirth as childbirth educator, founded Motherhealth, to serve seniors in the bay area with holistic caregivers and blogs at www.clubalthea.com with more than 10,000 health and finance related posts. Connie trains her own caregivers, which are the favorites of most bay area seniors who are home bound and alone. She is active in the rehab and nursing facilities, volunteering on music and movement for seniors. She is a member of Lion's club and offered scholarships to students in the Philippines. She is active at churchinsunnyvale.us and has Fridays Bible home study in Sunnyvale using the recovery version of the Bible , free at biblesforamerica.us She loves dancing and teaching and her courses can be found at https://teachclub.com/@thriveafter60 She is California Life Insurance licensed providing life insurance for older adults with health issues and helping women retire safely with income for life. at menloassetca.com , she helps with 401k rollover. 3 Benefit plans - Mortgage protection using term life insurance to pay for mortgage balance in event of death - Final Expense plan using Single Issue Whole Life Insurance, with cash back, disability benefit and guaranteed in the presence of health issues - Fixed Index Annuity retirement plan for safe, accessibility, less fees, less taxes, avoids probate as it goes directly to beneficiaries, rate of return with no downside market participation. She brings compassion and understanding to the needs of her clients, bringing holistic approach in health and life insurance. Her goal is to free families from worries especially during covid with caregivers and life insurance in the presence of health issues, especially for women. She can be reached at 408-854-1883 , motherhealth@gmail.com

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